Response of durum wheat mature embryo to callus induction and salt stress in vitro condition

Document Type : Original Article

Authors

1 1- Agricultural and Natural Resources Engineering Organization, Kermanshah, Iran, and MSc graduated in Plant Breeding, Faculty of Agricultural Sciences and Engineering, Razi University, Kermanshah, Iran

2 Dryland Agricultural Research Institute, Sararood Branch, Agricultural Research, Education and Extension Organization (AREEO), Kermanshah, Iran

Abstract

Introduction: Using plant tissue culture, including embryo culture, is one of the ways to increase genetic diversity to withstand environmental stresses, including salinity tolerance in plants.
Materials and methods: In this study, the reaction of 20 different durum wheat (Triticum turgidum var. durum Desf.) genotypes, including improved lines, native accessions, and two control cultivars, Zardak and Saji, were evaluated to the induction of callus formation and plant regeneration through mature embryo culture and comparison of callus response to salt stress condition. Murashige and Skoog (MS) medium was used for the mature embryo culture of durum wheat. For assessment of genotypes to salt stress, growing morphogenic calli were exposed to different concentrations of NaCl (0, 4, 8, 12, 16 and 20 dSm-1) added to the culture medium. The response of genotypes to in vitro NaCl stress was analyzed as a 20 × 6 factorial experiment based on a completely randomized design with three replications. Comparison of genotypes for callus induction from mature embryos was based on callus induction frequency, relative fresh weight growth of callus (RFWG), relative growth rate (RGR) and callus growth rate. The relative fresh weight growth of callus (RFWG), relative growth rate (RGR), callus growth rate and necrosis percent of callus were used for salt stress.
Results: The analysis of the correlation coefficients of the investigated traits in the conditions of callus induction showed that the callus induction percentage has a positive and significant correlation (P<0.01) with the callus growth rate. A positive and significant correlation (P<0.05) was also observed between the relative callus growth and the relative callus growth rate. Under salinity stress, relative callus growth showed a positive and significant correlation (P<0.01) with callus relative growth rate and a negative and significant correlation with callus chlorosis percentage. The results cluster analysis showed that genotypes 65-12-3-3, 75-5-3-5 and Zardak were classified in the same group of tolerant salt stress genotypes. Based on the results, Zardak variety and accessions 65-12-3-3 and 75-5-3-5 due to high relative growth rate and relative growth of callus and low chlorosis percentage in the saline environment were identified as tolerant genotypes to salinity under in vitro conditions. Genotype 25-25-1-5 had the highest response to adult embryo culture. The results showed a significant variation in the ability to callus induction in the genetic material under salinity stress conditions, which can be used in the durum wheat breeding program. It could be suggested to evaluate genotypes in field conditions and study the relationships between traits in vitro and in vivo.
Conclusion: Selected cells and plants provide a tool to determine the mechanisms involved in salt stress tolerance. The laboratory screening method for tolerance to salinity stress of plant genotypes can provide a suitable path for developing salinity-tolerant lines in durum wheat.

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Main Subjects

References

Abumhadi, N., Kamenarova, K., Todorovska, E., Dimov, G., Trifonova, A., Gecheff, K., & Atanassov, A. 2005. Callus induction and plant regeneration from barley mature embryos (Hordeum vulgare L.). Biotechnology and Biotechnological Equipment, 19 (3), 32-38.
Akhtar, N. 2006. Callogenesis and organogenesis response of wheat cultivars under sodium chloride salt stress. Pakistan Journal of Biological Sciences, 9(11), 2092-2096.
Ashraf, M., & Wu, L. 1994. Breeding for Salinity Tolerance in Plants. Critical Reviews in Plant Sciences, 13, 17-42.
Babu, S., Sheeba, A., Yogameenakshi, P., Anbumalarmathi, J., & Rangasamy, P. 2007. Effect of salt stress in the selection of salt tolerant hybridsin Rice (oryza sativa L.) under in vitro and in vivo condition. Asian journal of plant sciences, 6(1), 137-142.
Basu, S., Kumar, A., Benazir, I., & Kumar, G. 2021. Reassessing the Role of Ion Homeostasis for Improving Salinity Tolerance in Crop Plants. Physiologia Plantarum, 171, 502–519.
Birsin, M., & Ozgen, M. 2004. A comparison of callus induction and plant regeneration from different embryo explants of triticale (x triticosecale wittmack). Cellular and molecular biology letters, 9(2), 353-361.
Bradle, D.E., Bruneau, A.H., & Qu, R. 2001. Effects of cultivar explant treatment, and medium supplements on callus induction and plantlet regeneration in perennial ryegrass. International Turfgrass Society Research Journal, 9, 152-156.
EL-Sabagh, A., Hossain, A., Barutçular, C., Iqbal, M.A., Islam, M.S., Fahad, S., Sytar, O., Çig, F., Meena, R.S., & Erman, M. 2020. Consequences of Salinity Stress on the Quality of Crops and Its Mitigation Strategies for Sustainable Crop Production: An Outlook of Arid and Semi-Arid Regions. In: Environment, Climate, Plant and Vegetation Growth; Fahad, S., Hasanuzzaman, M., Alam, M., Ullah, H., Saeed, M., Ali Khan, I., Adnan, M., Eds.; Springer International Publishing: Cham, Switzerland, 2020; pp. 503–533. ISBN 978-3-030-49732-3.
Gamborg, O.L. 2002. Plant Tissue Culture. Biotechnology. Milestones. In Vitro Cellular & Developmental Biology - Plant, 38, 84-92.
Ganeshan, S., Baga, M., Harwey, B.L., Rossnagel, B.G., Scoles, G.J., & Chibbar, R.N. 2003. Production of multiple shoots from thiadiazuron- treated mature embryos and leaf- base/ apical meristems of barley (Hordeum vulgar l.). Plant Cell, Tissue and Organ Culture, 73, 57-64.
Grigoryeva, L.P., & Shletser, I.A. 2006. Screening wheat cultures for morphogenesis ability in immature embryo in vitro. Biologe 3-41.
Gonzalez, J.M., Friero, E., & Jouve, N. 2001. Influence of genotype and culture medium on callus formation and plant regeneration from immature embryos of Triticum turgidum Desf. Cultivars. Plant breeding, 120, 513-517.
Hagio, T., Ichiri, S.S., & Yamada, T. 2002. Efficient plant regeneration through morphogenesis in Japanese commercial variety of wheat. In Vitro Cellular and Developmental Biology – Plant, 38, 1394-1396.
Hess, J.R., & Carman, G. 1998. Embryogenic competence of immature wheat embryos: genotype, donor plant, environment, and endogenous hormone levels. Crop Science, 38, 249-253.
Jun-ying, C., Run-qing, Y., Hai-xian, X.U., & Xin-jian, C. 2006. Study on plant regeneration of wheat mature embryo under endosperm-supported culture. Agricultural Sciences in China, 5(8), 572-578.
Kacem, N.S., Delporte, F., Muhovski, Y., Djekoun, A., & Watillon, B. 2017. In vitro screening of durum wheat against water-stress mediated through polyethylene glycol. Journal of Genetic Engineering and Biotechnology, 15(1), 239-247.
Kumar, R., Mamrutha, H.M., Kaur, A., Venkatesh, K., Grewal, A., Kumar, R., & Tiwari, V. 2017 Development of an efficient and reproducible regeneration system in wheat (Triticum aestivum L.). Physiology and Molecular Biology of Plants, 23(4), 945-954.
Kumar, P.P., & Loh, C.S. 2012. Plant Tissue Culture for Biotechnology. In Plant Biotechnology and Agriculture; Elsevier: Amsterdam, The Netherlands, pp. 131-138.
Ihsanshah, M., Jabeen, M., & Ilahi, I. 2003. Invitro callus induction, its proliferation and regeneration in seed explants of wheat (Tritium aestivum l.). Pakistan Journal of Botany, 35(2), 209-217.
Isayenkov, S.V. 2019. Genetic Sources for the Development of Salt Tolerance in Crops. Plant Growth Regulation, 89, 1-17.
Kintzios, S.E., Baeberaki, M., Aivalakis, G., S., Drossopoulos, J., & Olevase, C.D. 1996. In vitro morphogenetical response of mature wheat embryo to differential NaCl concentration and growth regulator treatment. Plant Breeding, 166, 113-118.
Karadimova, M., & Djambpva, G. 1993. Increased NaCl- tolerance in wheat (Triticum aestivum L. and T. Durum Desf) through in vitro selection. In Vitro Cellular & Developmental Biology - Plant 29, 180-182.
 Al-Khayri, J.M., & Al-Bahrany, A.M. 2004. Growth, water content and proline accumulation in drought-stressed callus of date palm. Biologia Plantarum: 48(1). 105-108.
Maes, C.O., Chibbar, R.N., Caswell, K., Leung, N., & Kartha, K.K. (1996) Somatic embryogenesis from isolated scutella of wheat: effects of physical, physiological and genetic factors. Plant Science, 121, 75-84.
Munns, R., Day, D.A., Fricke, W., Watt, M., Arsova, B., Barkla, B.J., Bose, J., Byrt, C.S., Chen, Z.H., & Foster, K.J. 2020. Energy Costs of Salt Tolerance in Crop Plants. New Phycologist, 225, 1072-1090.
Ozgen, M., Turet, M., Ozcan, S., & Sancak, C. 1996. Callus induction and plant regeneration from immature and mature embryo of winter durum wheat genotypes. Plant Breeding, 15, 455-458.
Ozgen, M., Birsin, M., & Benlioglu, B. 2017. Biotechnological characterization of a diverse set of wheat progenitors (Aegilops sp. and Triticum sp.) using callus culture parameters. Plant Genetic Resources, 15(1), 45-50.
Ozturk, A., Bulut, S., Haliloglu, K., & Tosun, M. 2005. Relationship between tissue culture and agronomic traits of winter wheat. Cereal Research Communication, 33, 469-476.
Rashid, H., Ghani, R.A., Chaudhry, Z., Naqvi, S.M., & Quraishi, A. 2002. Effect of media, growth regulators and genotypes on callus induction and regeneration in wheat (Triticum aestivum L.). Biotechnology 1, 46-54.
Saldarriaga, J.F., Cruz, Y., & López, J.E. 2020. Preliminary study of the production of metabolites from in vitro cultures of C. ensiformis. BMC Biotechnology 20, 49.
Tripathi, A.D., Mishra, R., Maurya, K.K., Singh, R.B., & Wilson, D.W. 2019. Estimates for World Population and Global Food Availability for Global Health. In: The Role of Functional Food Security in Global Health; Elsevier: Amsterdam, The Netherlands, pp. 3–24.
Valoppi, F., Agustin, M., Abik, F., Morais de Carvalho, D., Sithole, J., Bhattarai, M., Varis, J.J., Arzami, A.N., Pulkkinen, E., & Mikkonen, K.S. 2021. Insight on Current Advances in Food Science and Technology for Feeding the World Population. Frontiers in Sustainable Food Systems, 5, 626227.
Wijerathna-Yapa, A., Ramtekey, V., Ranawaka, B., & Basnet, B.R. 2022. Applications of in Vitro Tissue Culture Technologies in Breeding and Genetic Improvement of Wheat. Plants, 11, 2273.  
Wijerathna-Yapa, A., & Hiti-Bandaralage, J. 2023. Tissue Culture—A Sustainable Approach to Explore Plant Stresses. Life, 13, 780.
Yu, Y., Wang, J., Zhu, M.L., & Wei, Z.M. 2008. Optimization of mature embryo based high frequency callus induction and plant regeneration from elite wheat cultivars grown in China. Plant Breeding, 127, 249-255.

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