نوع مقاله : مقاله پژوهشی
نویسندگان
1
گروه علوم کشاورزی دانشگاه پیام نور، تهران-ایران.
2
Professor, Medical Biology Research Center, University of Medical Sciences, Kermanshah-Iran.
3
گروه مهندسی تولید و ژنتیک گیاهی، پردیس کشاورزی و منابع طبیعی، دانشگاه رازی، کرمانشاه ، ایران.
10.22126/cbb.2025.11971.1105
چکیده
Introduction: Drought is one of the significant factors limiting wheat yield (Triticum aestivum L.). Understanding the morphological and physiological aspects of drought resistance is crucial in mitigating the problems caused by drought conditions. Drought limits photosynthesis and ultimately causes plant death; therefore, research on drought is beneficial. Drought stress plays a crucial role in physiological processes, metabolism, and the expression of several genes related to plant adaptation to drought stress.
Materials and Methods: To evaluate the effect of drought on protein profile, bread wheat (Rijaw cv) seedlings were grown in pots in a greenhouse. Drought stress was applied after the tillering stage and before the beginning of the stem stage, and its amount was considered to be 30% of Field Capacity. Leaf tissue samples were harvested 12 days after stress and immediately after being separated from the plant. Then, they were placed in aluminum foil and stored in liquid nitrogen until extraction. To compare the changes in leaf protein profiles between drought-treated and control plants, a proteomics method was employed. Leaf proteins were separated using the TCA-Acetone method and two-dimensional gel electrophoresis in the pH range of 4-10.
Results: Out of more than 400 protein spots reproducibly detected and analyzed, 29 spots showed significant changes under drought. Some proteins showed genotype-specific patterns of up- or down-regulation in response to drought. According to the results of variance analysis, chlorophyll a, chlorophyll b, and total chlorophyll were significantly different (P < 0.01) between the control sample and the other stress conditions, indicating a difference between the plants in the experimental treatments. Additionally, in terms of soluble protein traits, a significant difference exists between the treatments (P < 0.01), and this difference is evident in the expression of proteins across all treatments. Regarding the studied enzymes, peroxidase, superoxide dismutase, and carotenoids, a significant difference was observed between all the applied treatments (P < 0.01) and for the catalase enzyme (P < 0.05).
Conclusion: The results of mean comparison of all studied traits confirmed the significant difference; the data observed in this research showed that changes in antioxidant enzymes could provide a model to show the drought tolerance of wheat cultivars. Moreover, this research was able to identify the proteins involved in the tolerance of Rijaw cultivar wheat seedlings to drought stress, underscoring the importance of understanding the role of antioxidant enzymes in drought tolerance.
Keywords: Drought, 2D Electrophoresis, Peroxidase, Superoxide dismutase, Catalase, Carotenoids.
کلیدواژهها
موضوعات
عنوان مقاله [English]
Proteomic Profiling and Antioxidant Enzyme Activity Response to Drought Stress in Bread Wheat (Triticum aestivum) Rijaw Cultivar
نویسندگان [English]
-
Mehdi Kakaei
1
-
ali mostafaie
2
-
Mohsen Saeidi
3
1
Department of Agriculture, Payame noor university, Tehran-Iran.
2
Professor, Medical Biology Research Center, University of Medical Sciences, Kermanshah-Iran.
3
Department of Plant Production and Genetics, Campus of Agriculture and Natural Resources, Razi University, Kermanshah, Iran.
چکیده [English]
Drought is a major limitation to agricultural production worldwide. Drought stress plays an important role in physiological processes, metabolism and the expression of several genes that are related to plant adaptation to drought stress. Knowledge of the molecular and physiological mechanisms of drought reactions is necessary for the development of drought-tolerant and high-yielding genetic products . Drought stress has been identified as one of the factors limiting plant performance and a threat to the successful production of agricultural plants . Drought is one of the major factors limiting the yield of wheat (Triticum aestivum L.). To evaluate the effect of drought on protein profile, bread wheat (Rejaw cv) seedlings were grown in pots in a greenhouse. Drought stress was applied after tillering stage and before the beginning of the stem stage and its amount was considered to be 30% of Field Capacity (Three control treatments, under drought stress and after drought stress). Leaf tissue samples were harvested 12 days after stress and immediately after being separated from the plant. Then they were placed in aluminum foil and stored in liquid nitrogen until extraction. To compare the changes in leaf protein profile in drought treatment and control, the proteomics method was used. Leaf proteins were separated using the TCA-Acetone method and two-dimensional gel electrophoresis in the pH range of 4-10. Out of more than 400 protein spots reproducibly detected and analyzed, 29 spots showed significant changes under drought. Some proteins showed genotype-specific patterns of up- or down-regulation in response to drought. According to the results of variance analysis, chlorophyll a, chlorophyll b, and total chlorophyll were significant (P<0.01) between the control sample and other stress conditions, which indicates the difference between the plants in the experimental treatments. Also, in terms of soluble protein traits, there is a significant difference between the treatments (P<0.01), and this difference exists in the expression of proteins in all treatments. Regarding the studied enzymes, peroxidase, superoxide dismutase, and carotenoid, a significant difference was observed between all the applied treatments (P<0.01) and regarding the catalase enzyme (P<0.05). The results of comparing the average of all studied traits also confirmed the significant difference, the data observed in this research showed that changes in antioxidant enzymes can provide a model to show the drought tolerance of wheat cultivars. And of course, to some extent, this research was able to identify the proteins involved in the tolerance of Rejaw cultivar wheat seedlings to drought stress.
کلیدواژهها [English]
-
Drought
-
2D Electrophoresis
-
Peroxidase
-
Superoxide dismutase
-
Catalase
-
Carotenoids
)